Reconstitution

Lyophilized (freeze-dried) peptides arrive as a fine powder in the vial. Before use they must be reconstituted - mixed - by slowly adding a sterile liquid until the powder has fully dissolved.

How it works

Bacteriostatic water (BAC water) is the usual choice: the benzyl alcohol it contains inhibits microbes and keeps the solution stable for several weeks in the fridge. Inject the liquid down the inner glass wall - not straight onto the powder - then let the vial sit or swirl it gently. Do not shake: peptides are delicate protein chains and strong shear forces can damage them.

Common mistakes

• Vigorous shaking instead of gentle swirling.
• Spraying water directly onto the powder.
• Using the wrong water (e.g. tap water) - only sterile or bacteriostatic water.
• Not noting the volume added - you need the concentration (mg per ml) for dose math later.

Wording mix-ups

Reconstitution is often just called „mixing" or „dissolving"; sometimes people wrongly say „reconstructing". It always means the same thing: turning powder plus liquid into a ready-to-use solution.